The kynurenine (KYN) pathway (KP) of the tryptophan (TRP) metabolism seems to play a role in the pathomechanism of multiple sclerosis (MS). Cuprizone (CPZ) treated animals develop both demyelination (DEM) and remyelination (REM) in lack of peripheral immune response, such as the lesion pattern type III and IV in MS, representing primary oligodendrogliopathy.
To measure the metabolites of the KP in the CPZ treated animals, including TRP, KYN and kynurenic acid (KYNA). We proposed that KYNA levels might be decreased in the CPZ-induced demyelinating phase of the animal model of MS, which model represents the progressive phase of the disease.
A total of 64 C57Bl/6J animals were used for the study. Immunohistochemical (IHC) measurements were performed to prove the effect of CPZ, whereas high-performance liquid chromatography (HPLC) was used to quantify the metabolites of the KP (n = 10/4 groups; DEM, CO1, REM, CO2).
IHC measurements proved the detrimental effects of CPZ. HPLC measurements demonstrated a decrease of KYNA in the hippocampus (p &lt; 0.05), somatosensory cortex (p &lt; 0.01) and in plasma (p &lt; 0.001).
This is the first evidence of marked reduction in KYNA levels in a non-immune mediated model of MS. Our results suggest an involvement of the KP in the pathomechanism of MS, which needs to be further elucidated.
This is the first evidence of marked reduction in KYNA levels in a non-immune mediated model of MS. Our results suggest an involvement of the KP in the pathomechanism of MS, which needs to be further elucidated.There are growing campaigns to promote land titling to secure Land Tenure and Property Rights (LTPRs) in African agriculture. Theoretically, deed registration should reduce land disputes, facilitate land use as collateral for loans, and stimulate investment in land improvement for increased productivity, income and food security. Empirical evidence in these regards, however, remains anecdotal, and sometimes conflicting. This paper reports a study that examined LTPRs' among smallholder rice farmers in Northern Nigeria and the influence on household food security (HFS). It used cross-section data obtained from 549 rice farmers, selected by multistage sampling across 84 rice-growing communities, seven (7) States and the three (3) geopolitical zones in northern Nigeria. Data collection was by personal interviews of adult members of the farmers' households, focusing on the households' socio-economics, livelihoods, and LTPRs on farmland cultivated during the 2016/17 farming season. HFS was assessed within the framework of the United States Department of Agriculture' HFS Survey Module. LTPRs assessment was in terms of the type (source) and registration of titles to farmlands. HFS modelling was within the framework of Poisson, Instrumental Variable Poisson (IVP) and Zero-inflated Poisson (ZIP) regression methods, with endogeneity concerns and choice of specification addressed within Hausman specification tests. The results show that land titling is not endogenous in the estimated models; and that HFS is significantly (p less then 0.01) enhanced with an increase in shares of freehold and leasehold in the households' farmlands, as against reliance on communal holdings. Holding de jure secure title to farmlands, however, had no significant influence on HFS. The evidence supports the need to develop land markets to enhance the ease of land transfer, as part of measures to enhance HFS in northern Nigeria.Extensive use of atrazine as herbicide in crop farming in Nigeria may lead to its accumulation in fish feed ingredients or aquatic ecosystem from aerosol or by runoff resulting in its residue in aquatic animals. Atrazine residues were determined in fish feed and catfish (Clarias gariepinus) fillets from commercial aquaculture farms in Southwestern Nigeria by matrix solvent particle dispersion and quantification using an ELISA kit. The mean atrazine concentrations in feed and fish were about 1.3-1.5 μg/kg and 1.4-1.8 μg/kg respectively. Atrazine was mostly detected in catfish from Ogun State (91.3%) and feed from Lagos State (80.0%) with mean concentrations of 1.4 ± 0.4 μg/kg and 1.5 ± 0.5 μg/kg, respectively. Mean atrazine concentration in catfish samples from Lagos State was significantly higher (P less then 0.05) than the mean concentration in catfish samples from Ogun State. This study showed that the Estimated Average Daily Intake (EADI) of atrazine in fish samples from the selected states were below the Acceptable Daily Intake (ADI) value of 6 μg/kg for herbicide residues and thus within safe limit but their presence in fish is a cause for concern.Cardiovascular computed tomography (cardiovascular CT) is currently used as a fast non-invasive method for the visualization of coronary plaques and walls and the assessment of lumen stenosis severity. https://www.selleckchem.com/ Previous studies demonstrated the high negative predictive value of CT for the exclusion of coronary lumen stenoses. In this study we hypothesize that coronary CT angiography (CTA) represents a reliable method as diagnostic procedure in acute coronary syndrome (ACS) even in emergency settings.
36 patients (51 lesions) with ACS who underwent cardiovascular CT, intravascular ultrasound (IVUS) and invasive coronary angiography (ICA) within 48 h were included. The percentage of coronary stenoses were measured and compared by three methods. Influence of available predictors that can potentially affect the measurement results was assessed.
Cardiac CTA provided comparable results to IVUS (mean difference -0.45%, PPV 98%, NPV 75%). ICA tends to estimate lower stenoses degrees than cardiac CTA and IVUS (mean difference 13.19% and 13.64%, respectively). The final diagnosis and positive remodeling did not lead to any significant influence on measurements.
The cardiovascular CT results show that even in emergency settings it is possible to identify morphological changes as sequels of coronary artery sclerosis with comparable results to the reference method IVUS. Deviations of IVUS and cardiovascular CT from ICA are comparable and can to a large extent be explained by differences in the measurement technique.
The cardiovascular CT results show that even in emergency settings it is possible to identify morphological changes as sequels of coronary artery sclerosis with comparable results to the reference method IVUS. Deviations of IVUS and cardiovascular CT from ICA are comparable and can to a large extent be explained by differences in the measurement technique.